1. Mr. Freeze
Because Mr. Freeze in this film is considered one of the worst portrayals of villains of all time, they decided not to have him in anything else for several years.
Bane another villain from the movie. Greatly mocked for his portrayal, Robert Swenson died just one month after the release date of Batman and Robin, his last film.
3. Poison Ivy
The third villain from the film, Poison Ivy, whose origin was similar to her comic book counterpart, but not exact.
In the Pre-Crisis version the identity was Dr. Lillian Rose, a promising botanist from Seattle who is seduced by Marc LeGrande to help him steal a mysterious Egyptian plant from a museum. He then poisons her with the plant to keep her from telling anyone. She then discovers the plant has given her an immunity to all toxins.
In the Post-Crisis version the identity was Pamela Isley who is seduced by her college professor Dr. Jason Woodrue into letting him use experimental poisons and toxins causing her to develop abilities to control, manipulate, and grow plants.
In the New 52, as a kid, Pamela Isley had a skin disease that forced her into the confines of her house, but when she was outside she loved the garden. At some point she watched her abusive father eventually kill her mother, and then bury her in the garden. While in college, Pamela sold pheromone pills to other students to study its effects until she was caught by police. She used a powerful version of the pills to mindcontrol the Dean so he would drop the charges and let her graduate from college with honors. While visiting her father in prison, she kissed him with a poison that was secreted from her lips that killed him. Later she landed an internship in Wayne Enterprises in its Bio-Chem division developing pharmaceutical and cosmetic applications. She was fired after proposing Bruce Wayne to develop chemicals that trigger social or behavioral responses (basically brainwashing). As she was escorted out by security, she accidentally spilled the chemicals she was working with on herself, giving her powers to control plant life and immunity to all poisons and viruses.
In Batman Forever, Dick Grayson attempted to become Batman's sidekick by listing possible names for himself including bat boy, and Nightwing. In the comics, Dick Grayson was the first Robin and when he rebelled against Batman, he become his own hero named Nightwing.
In Batman and Robin one of the new suits Dick wears has the symbol of Nightwing instead of Robin.
The Batmobile in this movie was one of the longest, it was completely custom using many different elements of many different cars.
6. Julie Madison
The character had far fewer parts in the finished film, but in the comics was the first and one of the biggest loves of Bruce's life.
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